Isolation of Ribosome-like Structures from Pichinde Virus

Isolation of virus like particles from fecal material obtained from diarrheal calves

Polymerase activity of Pichinde virus.

Pichinde virus, a member of the arenavirus group, was examined for polymerase activity. Purified virus was found to contain RNA-dependent RNA polymerase but not RNA-dependent DNA polymerase activity. Since RNase but neither DNase nor actinomycin D inhibited the endogenous polymerase reaction, RNA of the virus appeared to be used as the template. The divalent cations Mg(2+) and Mn(2+) were requi...

Strandedness of Pichinde virus RNA.

The Pichinde virus RNA did not possess the following characteristics of eucaryotic mRNA: polyadenylic acid sequence, capped methylated structure, and ability to direct protein synthesis in vitro. Polysomal RNA extracted from cells infected with Pichinde virus reannealed with 32P-labeled virus RNA, protecting about 60% of the latter against RNase degestion. The polyadenylic acid-containing polys...

Isolation and Identification of the Infectious Haematopoietic Necrosis Virus (IHNV)-like Agent from Farmed Rainbow Trout (Oncorhynchus mykiss) from Iran

P3: Isolation of Neurosphere-Like Bodies from Adult Human Amygdala

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